Description
This is a user-friendly and comprehensive treatise on enzyme kinetics - indispensable for biochemists, biologists, medical scientists, and chemists working with enzymes, from advanced students to experts in academia and industry. Theory and practice are well-balanced, the relation to the biological system is always emphasized. Theoretical aspects are presented in a way which is also comprehensible for the beginner. An extensive methodological part provides the expert with valuable support in planning and performing laboratory experiments. Contains a CD-ROM with EKI-3, the elaborate and easy-to-use version of the enzyme kinetics practical course.
Read More
By applying techniques to pursue rapid kinetic reactions it is possible to separate a complete reaction into different time segments and to determine the rate constants of individual steps of the reaction. The author demonstrates the methods by which this is done and explains the theory behind it. Topics include reversible enzyme reactions, enzyme inhibition, multi-substrate reactions, special enzyme mechanisms, and isotope exchange. Specific methods detailed include electrochemical methods, calorimetry, and spectroscopy. The CD-ROM contains a graphics-plotting program intended as a companion to the book. Translated from the third edition of the German work Enzymkinetik Theorie und Methoden . Annotation c. Book News, Inc., Portland, OR (booknews.com)
Read Less