Fluorescence Spectroscopy (Volume 278) (Methods in Enzymology, Volume 278)
Books / Hardcover
Books › Science › Spectroscopy & Spectrum Analysis
ISBN: 012182179X / Publisher: Academic press, June 1997
Details methods and applications in fluorescence spectroscopy, focusing on the fluorescence of tryptophan, the origin of excited states, and their sensitivity to environmental perturbations. Presents research on constrained tryptophan residues, tryptophan in crystals, and fluorescence of proteins, and examines applications of fluorescence to studies of nucleic acids and protein-nucleic acid interactions. Other subjects include energy transfer and emission anisotrophy, measurement of DNA cleavage, time-dependent protein phosphorescence, and applications of fluorescence to studies of bilayer membranes. Annotation c. by Book News, Inc., Portland, Or.
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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features* Fluorescence in biophysics* Fluorescence as a tool for studying proteins* Determination of ground-state dissociation constant by fluorescence spectroscopy* Fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions* Fluorescence methods for studying equilibrium macromolecule-liquid interactions* Methods for detecting molecular clusters on cell surfaces
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