The Protein Protocols Handbook (Methods in Molecular Biology)
Books / Paperback
ISBN: 0896039412 / Publisher: Humana Press, February 2002
This second edition of a collection of 164 reproducible protocols introduces topics and techniques that have emerged in the field since 1996, especially the application of mass spectrometry in protein characterization and the 2-D PAGE technique in proteomics. A section on glycoprotein is substantially expanded, and methods for the production of single chains and phage-displayed antibodies have been added. Each method offers a concise summary of its basic theory, a materials list, a step-by-step protocol, and notes on avoiding pitfalls and modifying the method to individual circumstances. Most authors are the originators of the techniques described. Walker is affiliated with the University of Hertfordshire, UK. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Read More
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular Biology™ series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Read Less