Description
New edition of a collection of immunocytochemical methods, divided into sections on: antibody preparation; light microscopic detection systems; fluorescence-activated cell sorter analyses; colloidal gold detection systems for electron microscope analysis; and applications for nucleic acid localization by in situ hybridization. The 52 methods presented are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. The protocols are designed to be adaptable and thus applicable within a wide variety of experimental systems. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.
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Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
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