Description
In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene or the biochemistry of a new transcription factor.This book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.
Read More
After a review of the state-of-the-art in the RNA polymerase II transcription field, subsequent chapters present a systematic guide to the investigation of regulatory mechanisms for newly identified genes, and discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering their mode of action. Carey (UCLA) and Smale (Howard Huges Medical Institute and UCLA) write for graduate students, physician-scientists, and others, either beginning or already established, who are undertaking in-depth studies in the analysis of gene expression in model systems or disease states. They put methods in the context of underlying theory, recommend experimental strategies, encourage creativity in investigative design, and explain protocols, with troubleshooting advice. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Read Less