Description
mRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed. Key Features* Isolation and characterization of specific RNA binding proteins* RNA metabolism and associated regulatory proteins* RNA detection and localization* A genetic approach to RNA function
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This volume deals with new methods used in RNA research to accomplish such tasks as the isolation and characterization of specific RNA binding proteins; RNA metabolism and associated regulatory proteins; mRNA detection and localization; and genetic approaches to the study of RNA function. Methods discussed include in vitro analysis of mammalian cell mRNA 3' processing; interactions of proteins with specific sequences in RNA; techniques for studying gene expression in Drosophila melanogaster tissue; and differential display protocol that preferentially identifies mRNA of moderate to low abundance in a microscopic system. Some b&w illustrations and a couple of color plates. Annotation c. by Book News, Inc., Portland, Or.
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